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FB2026_01
,
released March 12, 2026
Source was adult ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was adult ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
In the presence of RNase during immunoprecipitation this interaction was not observed, indicating that it is probably indirect and mediated through RNA in the RNP complex.
Source was adult ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
In the presence of RNase during immunoprecipitation this interaction was not observed, indicating that it is probably indirect and mediated through RNA in the RNP complex.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was live S2 cells; fluorescence donor produced from transfected construct; fluorescence acceptor produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction carried out in the presence of eIF4E3.
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.