FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
EndoA, Synj
FlyBase ID
FBig0000098369
Interaction Network
Interactions Browser links
EndoA network
Synj network
Reported Interactions
FBrf0167964-1.PD.WB
Reference
Verstreken et al., 2003
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Synj 

Synj

bait

GST tag

Synj 
EndoA 

Endo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Synj 
proline-rich domain
sufficient binding region
aa 1-301
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult head extract.

(Verstreken et al., 2003)

The proline-rich domain of synj interacts with endoA and Dap160, but not to shi or n-syb.

(Verstreken et al., 2003)
FBrf0167964-5.coIP.WB
Reference
Verstreken et al., 2003
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EndoA 

Endo

bait
Synj 

Synj

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Verstreken et al., 2003)
FBrf0225491-4.coIP.WB
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
adult head / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Synj 

Synj

bait
EndoA 

Endo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Synj-EndoA interaction is increased in a mnb mutant background.

(Chen et al., 2014)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chen et al., 2014)
FBrf0225491-7.coIP.WB
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
adult head / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EndoA 

Endo

bait
Synj 

Synj

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The EndoA-Synj interaction is increased in a mnb mutant background.

(Chen et al., 2014)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chen et al., 2014)
FBrf0233267-3.coIP.WB
Reference
Geng et al., 2016
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Synj 

Synj-1

bait

HA tag

Synj 
Synj 
EndoA 

Endophilin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Synj 
phosphorylation target S1029
mutation disrupting interaction
aa 1029

S1029E, coordinates relative to Synj-PA

Synj 
phosphorylation target S1029
mutation increasing interaction
aa 1029

S1029A, coordinates relative to Synj-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Geng et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)