FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fzy, mad2
FlyBase ID
FBig0000098370
Interaction Network
Interactions Browser links
fzy network
mad2 network
Reported Interactions
FBrf0210988-1.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
fzy 

Cdc20

bait
mad2 

Mad2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The fzy-mad2 interaction is increased upon metaphase arrest via Cdc27 depletion.

(Li et al., 2010)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2010)
FBrf0210988-3.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzy 

Cdc20

bait
mad2 

Mad2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2010)
FBrf0210988-5.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fzy 

Cdc20

bait

GFP tag

mad2 

Mad2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2010)

The fzy-mad2 interaction is increased upon metaphase arrest via MG132 treatment.

(Li et al., 2010)
FBrf0221865-2.coIP.WB
Reference
Conde et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
fzy 

Cdc20

bait
mad2 

Mad2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cells were treated with MG132 for one hour, then two hours with colchicine, to induce the formation of the mitotic checkpoint complex (MCC).

(Conde et al., 2013)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Conde et al., 2013)

The fzy-mad2 interaction is reduced in cells depleted of polo activity by RNAi or the inhibitor BI2536, suggesting a role for polo in the spindle assembly checkpoint.

(Conde et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)