FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Shc, Egfr
FlyBase ID
FBig0000098397
Interaction Network
Interactions Browser links
Shc network
Egfr network
Reported Interactions
FBrf0083246-2.coIP.WB
Reference
Lai et al., 1995
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shc 

dShc

bait
Egfr 

DER

prey

activated Egfr chimeric protein

Egfr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Egfr 
cytoplasmic domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic flies carried a heat-inducible transgene encoding an activated tor-Egfr chimeric protein.

(Lai et al., 1995)

Shc binds to activated, tyrosine-phosphorylated Egfr.

(Lai et al., 1995)

Source was adults of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.

(Lai et al., 1995)
FBrf0083246-3.PD.WB
Reference
Lai et al., 1995
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shc 

dShc

bait

GST tag

Shc 
Egfr 

DER

prey

activated Egfr chimeric protein

Egfr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shc 
PTB domain
sufficient binding region
aa 1-203
Egfr 
cytoplasmic domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic flies carried a heat-inducible transgene encoding an activated tor-Egfr chimeric protein.

(Lai et al., 1995)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic fly extract.

(Lai et al., 1995)
FBrf0222556-1.PD.WB
Reference
van der Geer et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shc 

dShc

bait

GST tag

Shc 
Shc 
Egfr 

DER

prey

activated Egfr chimeric protein

Egfr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shc 
PTB domain
sufficient binding region
aa 1-203
Shc 
PTB domain
mutation disrupting interaction
aa 151

R151A

Egfr 
cytoplasmic domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic flies carried a heat-inducible transgene encoding an activated tor-Egfr chimeric protein.

(van der Geer et al., 1996)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic fly extract.

(van der Geer et al., 1996)
FBrf0222949-1.coIP.WB
Reference
Bianco et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Egfr 

EGFR

bait

FLAG tag

Egfr 
Shc 

Shc

prey

HA tag

Shc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shc 
PTB-SH2 domains
sufficient binding region
Egfr 
intracellular domain
mutation disrupting interaction
aa 1357

Y1357F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bianco et al., 2007)
FBrf0222949-16.Y2H
Reference
Bianco et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Egfr 

EGFR

bait
Egfr 
Egfr 
Shc 

Shc

prey
Shc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Egfr 
intracellular domain
sufficient binding region
Egfr 
intracellular domain
mutation disrupting interaction
aa 1357

Y1357F

Shc 
PTB domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast

(Bianco et al., 2007)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bianco et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)