polybromo can immunoprecipitate brm and mor, but not osa, suggesting that osa and polybromo exist in distinct Brahma complexes.

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

polybromo is present with brm, mor and Bap170 in a distinct Brahma complex that lacks osa.

Source was embryonic nuclear extract wild-type fly line; proteins produced from endogenous gene.

Nuclear extract was subjected to several chromatographic steps to isolate brm-containing complexes.

BAP complex purified via Bap60-HA immunoprecipitation, cross-links detected by mass spectrometry.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.