FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
inaD, ninaC
FlyBase ID
FBig0000098441
Interaction Network
Interactions Browser links
inaD network
ninaC network
Reported Interactions
FBrf0108298-1.PD.A
Reference
Wes et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ninaC 

NINAC

bait

GST tag

ninaC 
inaD 

INAD

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ninaC 
C terminal domain
sufficient binding region
aa 1248-1501

coordinates relative to ninaC-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Wes et al., 1999)
FBrf0108298-2.Y2H
Reference
Wes et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

fused to GAL4 DNA-binding domain

ninaC 

ninaC

prey

fused to GAL4 activation domain

ninaC 
ninaC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ninaC 
PDZ1 domain
sufficient binding region
aa 1-145
ninaC 
C terminal isoleucine residue 1501
necessary binding region
aa 1501

I1501E coordinates relative to ninaC-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Wes et al., 1999)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Wes et al., 1999)
FBrf0108298-3.coIP.WB
Reference
Wes et al., 1999
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait
ninaC 

nincC

prey
ninaC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ninaC 
C terminal isoleucine residue 1501
necessary binding region
aa 1501

I1501E coordinates relative to ninaC-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads from wild type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wes et al., 1999)
FBrf0221262-8.Y3H
Reference
Mazzotta et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ninaC 

NinaC

bait

fused to B42 activation domain

inaD 

inaD

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three-hybrid system: yeast cry-LexA-BD/ninaCB42-AD/inaD-FLAG.

(Mazzotta et al., 2013)

inaD binds ninaC-B42 (transcriptional activation domain) to bridge the interaction with cry-LexA (DNA binding domain) and activate the reporter.

(Mazzotta et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Mazzotta et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)