FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Klc, Aplip1
FlyBase ID
FBig0000098452
Interaction Network
Interactions Browser links
Klc network
Aplip1 network
Reported Interactions
FBrf0149603-12.coIP.WB
Reference
Taru et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klc 

DKLC

bait

FLAG tag

Klc 
Aplip1 

APLIP1

prey

HA tag

Aplip1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Aplip1 
C-terminal region
necessary binding region
aa 354-490

\'CΔ137\'

Klc 
region
sufficient binding region
aa 162-508
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Taru et al., 2002)
FBrf0190020-1.coIP.WB
Reference
Horiuchi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Klc 

KLC

bait

MYC tag

Aplip1 

APLIP1

prey

FLAG tag

Aplip1 
Aplip1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Aplip1 
KBD domain
necessary binding region
aa 480-490

coordinates relative to Aplip1-PB

Aplip1 
KBD domain
mutation decreasing interaction
aa 483aa 483

P483A, coordinates relative to Aplip1-PB

P483L, coordinates relative to Aplip1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Horiuchi et al., 2005)
FBrf0201414-3.coIP.WB
Reference
Horiuchi et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Klc 

Klc

bait

MYC tag

Aplip1 

APLIP1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Horiuchi et al., 2007)

Co-precipitation of Aplip1 with Klc was partially inhibited by expression of wild-type hep and was more completely inhibited by a constitutively active mutant hep. Near-complete inhibition was also observed with coexpression of wild-type wnd and wild-type hep.

(Horiuchi et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)