FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cry, ninaC
FlyBase ID
FBig0000098486
Interaction Network
Interactions Browser links
cry network
ninaC network
Reported Interactions
FBrf0221262-2.coIP.MS
Reference
Mazzotta et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

CRY

bait

HA tag

ninaC 

NinaC

prey

1 unique peptide identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Flies were raised in light:dark cycles, and collected after a 15 minute light pulse.

(Mazzotta et al., 2013)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mazzotta et al., 2013)
FBrf0221262-3.coIP.WB
Reference
Mazzotta et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

CRY

bait

HA tag

ninaC 

NinaC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The ninaC-cry interaction is stronger in extracts obtained from flies during the light phase of their light:dark cycle.

(Mazzotta et al., 2013)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mazzotta et al., 2013)
FBrf0221262-6.Y3H
Reference
Mazzotta et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

dCRY

bait

fused to LexA DNA-binding domain

ninaC 

NinaC

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three-hybrid system: yeast LexA-BD/B42-AD, with inaD expression.

(Mazzotta et al., 2013)

In this assay, cry and ninaC do not interact directly, but instead require inaD to bridge the interaction.

(Mazzotta et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Mazzotta et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)