FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dpr11, DIP-γ
FlyBase ID
FBig0000098582
Interaction Network
Interactions Browser links
dpr11 network
DIP-γ network
Reported Interactions
FBrf0222867-133.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
DIP-γ 

CG14521

bait

Fc tag

DIP-γ 
dpr11 

dpr11

prey

alkaline phosphatase tag, COMP pentamerization motif

dpr11 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DIP-γ 
extracellular domain
sufficient binding region
dpr11 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
FBrf0222867-47.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
dpr11 

dpr11

bait

Fc tag

dpr11 
DIP-γ 

CG14521

prey

alkaline phosphatase tag, COMP pentamerization motif

DIP-γ 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dpr11 
extracellular domain
sufficient binding region
DIP-γ 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
FBrf0230454-116.SPR
Reference
Carrillo et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dpr11 

Dpr11

bait
DIP-γ 

DIP-γ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected High Five cell system; prey produced as a recombinant fusion protein in baculovirus-infected High Five cell system.

(Carrillo et al., 2015)

Kd = 4.0 uM

(Carrillo et al., 2015)
FBrf0230454-38.ELISA
Reference
Carrillo et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
dpr11 

dpr11

bait

Fc tag

DIP-γ 

DIP-γ

prey

alkaline phosphatase tag, COMP pentamerization motif

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Carrillo et al., 2015)
FBrf0230454-95.ELISA
Reference
Carrillo et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
DIP-γ 

DIP-γ

bait

Fc tag

dpr11 

dpr11

prey

alkaline phosphatase tag, COMP pentamerization motif

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Carrillo et al., 2015)
FBrf0241075-22.SPR
Reference
Cosmanescu et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-γ 

DIP-γ

bait
DIP-γ 
dpr11 

Dpr11

prey
dpr11 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DIP-γ 
extracellular domain
sufficient binding region
aa 22-358
dpr11 
extracellular domain
sufficient binding region
aa 114-324

coordinates relative to dpr11-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in HEK293F cells; prey produced as a recombinant fusion protein in HEK293F cells.

(Cosmanescu et al., 2018)

Kd = 8.97uM

(Cosmanescu et al., 2018)
FBrf0241500-2.XC
Reference
Cheng et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dpr11 

Dpr11

neutral component
dpr11 

Dpr11

neutral component
dpr11 
dpr11 

Dpr11

neutral component
dpr11 
DIP-γ 

DIP-γ

neutral component
DIP-γ 
DIP-γ 

DIP-γ

neutral component
DIP-γ 

DIP-γ

neutral component
DIP-γ 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dpr11 
IG1 domain
sufficient binding region
unspecified
DIP-γ 
IG1,IG2 domains
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculoviral Sf9 cell system.

(Cheng et al., 2019)
FBrf0241500-5.SPR
Reference
Cheng et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dpr11 

Dpr11

bait
dpr11 
DIP-γ 

DIP-γ

prey
DIP-γ 
DIP-γ 
DIP-γ 
DIP-γ 
DIP-γ 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dpr11 
IG1 domain
sufficient binding region
unspecified
DIP-γ 
IG1,IG2 domains
sufficient binding region
unspecified
DIP-γ 
IG1 domain residue 79
mutation decreasing interaction
aa 79

T79A

DIP-γ 
IG1 domain residue 80
mutation decreasing interaction
aa 80

V80A

DIP-γ 
IG1 domain residue 88
mutation decreasing interaction
aa 88

V88A

DIP-γ 
IG1 domain residue 122
mutation decreasing interaction
aa 122

Q122A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion proteins in baculoviral Sf9 cell system; prey produced as a recombinant fusion proteins in baculoviral Sf9 cell system.

(Cheng et al., 2019)
FBrf0241500-6.SPR
Reference
Cheng et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-γ 

DIP-γ

bait
DIP-γ 
dpr11 

Dpr11

prey
dpr11 
dpr11 
dpr11 
dpr11 
dpr11 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dpr11 
IG1 domain
sufficient binding region
unspecified
DIP-γ 
IG1,IG2 domains
sufficient binding region
unspecified
dpr11 
IG1 domain residue 158
mutation decreasing interaction
aa 158

H158A

dpr11 
IG1 domain residue 159
mutation decreasing interaction
aa 159

I159A

dpr11 
IG1 domain residue 167
mutation decreasing interaction
aa 167

F167A

dpr11 
IG1 domain residue 201
mutation decreasing interaction
aa 201

Q201A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion proteins in baculoviral Sf9 cell system; prey produced as a recombinant fusion proteins in baculoviral Sf9 cell system.

(Cheng et al., 2019)
FBrf0245556-3.SPR
Reference
Sergeeva et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-γ 

DIP-γ

bait
DIP-γ 
dpr11 

Dpr11

prey
dpr11 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DIP-γ 
Ig1 domain, residue 77
mutation decreasing interaction
aa 77

D77T, coordinates relative to DIP-γ-PA

dpr11 
Ig1 domain, residues 205,207
mutation decreasing interaction
aa 205207

E205Q,K207V

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from transfected HEK293F cells; prey purified from transfected HEK293F cells.

(Sergeeva et al., 2020)

Kd = 7.9uM, average of 5 independent measurements

(Sergeeva et al., 2020)
FBrf0258738-4.BA.FM
Reference
Lobb-Rabe et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-γ 

DIP-γ

neutral component

EGFP tag

DIP-γ 

DIP-γ

neutral component

EGFP tag

DIP-γ 

DIP-γ

neutral component

EGFP tag

dpr11 

dpr11

neutral component

mScarlet tag

dpr11 

dpr11

neutral component

mScarlet tag

dpr11 

dpr11

neutral component

mScarlet tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live Sf9 insect cells; proteins produced from baculovirus constructs.

(Lobb-Rabe et al., 2024)

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

(Lobb-Rabe et al., 2024)

Assay: cell aggregation. Addition of phospholipase-C disrupts interaction.

(Lobb-Rabe et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)