FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
BubR1, Cdc27
FlyBase ID
FBig0000098645
Interaction Network
Interactions Browser links
BubR1 network
Cdc27 network
Reported Interactions
FBrf0221865-8.coIP.WB
Reference
Conde et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
BubR1 

BubR1

bait
Cdc27 

Cdc27

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cells were treated with MG132 for one hour, then two hours with colchicine, to induce the formation of the mitotic checkpoint complex (MCC).

(Conde et al., 2013)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Conde et al., 2013)

The BubR1-Cdc27 interaction is not observed in cells depleted of mad2 by RNAi. Depletion of ald or Ndc80/Nuf2 has no effect.

(Conde et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)