FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pvr, Vav
FlyBase ID
FBig0000098665
Interaction Network
Interactions Browser links
Pvr network
Vav network
Reported Interactions
FBrf0221764-1.coIP.WB
Reference
Fernández-Espartero et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Pvr 

PVR

bait
Vav 

DroVav

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fernández-Espartero et al., 2013)
FBrf0221764-2.coIP.WB
Reference
Fernández-Espartero et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Vav 

DroVav

bait
Pvr 

PVR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fernández-Espartero et al., 2013)
FBrf0221764-3.Y2H
Reference
Fernández-Espartero et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pvr 

PVR

bait
Pvr 
Pvr 
Pvr 
Vav 

VAV

prey
Vav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pvr 
intracellular domain
sufficient binding region
Pvr 
intracellular domain
necessary binding region
aa 1261-1352

\'Δ313\' construct

Pvr 
intracellular domain tyrosine residues
mutation decreasing interaction
aa 994-1495

14 Tyr-Phe mutations, \'YF14\' construct

Vav 
SH2 and SH3 domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

(Fernández-Espartero et al., 2013)

Two-hybrid system: yeast

(Fernández-Espartero et al., 2013)
FBrf0222949-5.Y2H
Reference
Bianco et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pvr 

PVR

bait
Pvr 
Pvr 
Vav 

Vav

prey
Vav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pvr 
intracellular domain
sufficient binding region
Pvr 
kinase domain
mutation disrupting interaction

kinase dead mutant shows no interaction

Vav 
SH2-SH3 domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast

(Bianco et al., 2007)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bianco et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)