FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
X11L, Appl
FlyBase ID
FBig0000098685
Interaction Network
Interactions Browser links
X11L network
Appl network
Reported Interactions
FBrf0149072-1.Y2H
Reference
Hase et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Appl 

APPL

bait

fused to GAL4 DNA-binding domain

Appl 
X11L 

dX11L

prey

fused to GAL4 activation domain

X11L 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Appl 
cytoplasmic domain
sufficient binding region
aa 834-886
X11L 
PI and PDZ domains
sufficient binding region
aa 731-1168
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hase et al., 2002)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Hase et al., 2002)
FBrf0149072-2.PD.WB
Reference
Hase et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Appl 

APPL

bait

GST tag

Appl 
X11L 

dX11L

prey

Myc tag

X11L 
X11L 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Appl 
cytoplasmic domain
sufficient binding region
aa 834-886
X11L 
PI and PDZ domains
sufficient binding region
aa 778-1168
X11L 
phosphotyrosine interaction (PI) domain
necessary binding region
aa 778-935
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected COS7 cell extract.

(Hase et al., 2002)
FBrf0187897-2.coIP.WB
Reference
Ashley et al., 2005
Description
physical association
Assay
Collection
Source/Stage
larval somatic muscle cell / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
X11L 

dX11

bait
Appl 

APPL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval body wall muscle of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ashley et al., 2005)
FBrf0187897-4.coIP.WB
Reference
Ashley et al., 2005
Description
physical association
Assay
Collection
Source/Stage
larval somatic muscle cell / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
X11L 

dX11

bait
Appl 

APPL

prey
Appl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Appl 
cytoplasmic internalization sequence
necessary binding region
GYENPTY
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval body wall muscle of wild-type fly line; bait produced from endogenous gene; prey produced from transgenic construct.

(Ashley et al., 2005)
FBrf0187897-5.coIP.WB
Reference
Ashley et al., 2005
Description
physical association
Assay
Collection
Source/Stage
larval somatic muscle cell / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
X11L 

dX11

bait
X11L 
Appl 

APPL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
X11L 
PTB domain
necessary binding region
aa 766–986
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval body wall muscle of wild-type fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Ashley et al., 2005)
FBrf0187897-7.coIP.WB
Reference
Ashley et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Appl 

APPL

bait
X11L 

dX11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Ashley et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)