FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098711
Interaction Network
Interactions Browser links
side-IV network
beat-IIb network
Reported Interactions
FBrf0222867-111.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CG14372

bait

Fc tag

beat-IIb

prey

alkaline phosphatase tag, COMP pentamerization motif

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
extracellular domain
sufficient binding region
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

FBrf0222867-25.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

beat-IIb

bait

Fc tag

CG14372

prey

alkaline phosphatase tag, COMP pentamerization motif

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
extracellular domain
sufficient binding region
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

FBrf0258900-1.BA.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Side-IV

neutral component

mCherry tag

Side-IV

neutral component

mCherry tag

Side-IV

neutral component

mCherry tag

Beat-IIb

neutral component

GFP tag

Beat-IIb

neutral component

GFP tag

Beat-IIb

neutral component

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ig 1-5 domains
necessary binding region
unspecified

coordinates relative to side-IV-PC

Ecto-delta-FnIII domain
mutation increasing interaction
unspecified

coordinates relative to side-IV-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

Assay: cell aggregation

FBrf0258900-4.BA.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Side-IV

neutral component

GFP tag

Side-IV

neutral component

GFP tag

Side-IV

neutral component

GFP tag

Beat-IIb

neutral component

V5 tag

Beat-IIb

neutral component

V5 tag

Beat-IIb

neutral component

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PDZb domain
mutation decreasing interaction
unspecified

coordinates relative to side-IV-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

Assay: cell aggregation

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)