FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cry, inaD
FlyBase ID
FBig0000098714
Interaction Network
Interactions Browser links
cry network
inaD network
Reported Interactions
FBrf0221262-4.coIP.WB
Reference
Mazzotta et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

CRY

bait

HA tag

inaD 

inaD

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The inaD-cry interaction is stronger in extracts obtained from flies during the light phase of their light:dark cycle.

(Mazzotta et al., 2013)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mazzotta et al., 2013)
FBrf0221262-5.Y2H
Reference
Mazzotta et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

dCRY

bait

fused to LexA DNA-binding domain

cry 
cry 
inaD 

INAD

prey

fused to B42 activation domain

inaD 
inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
calmodulin binding motif and PDZ domains 2 and 3
sufficient binding region
aa 207-448
inaD 
calmodulin binding motif
necessary binding region
aa 207-248
cry 
C-terminal region
sufficient binding region
aa 521-540
cry 
C-terminal region
necessary binding region
aa 521-540
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Mazzotta et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Mazzotta et al., 2013)
FBrf0221262-7.Y3H
Reference
Mazzotta et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

dCRY

bait

fused to LexA DNA-binding domain

inaD 

inaD

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three-hybrid system: yeast cry-LexA-BD/ninaCB42-AD/inaD-FLAG.

(Mazzotta et al., 2013)

inaD binds cry-LexA (DNA binding domain) to bridge the interaction with ninaC-B42 (transcriptional activation domain) and activate the reporter.

(Mazzotta et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Mazzotta et al., 2013)
FBrf0239985-2.coIP.WB
Reference
Mazzotta et al., 2018
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

dCRY

bait

HA tag

inaD 

inaD

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The cry-inaD interaction is strengthened by light.

(Mazzotta et al., 2018)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mazzotta et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)