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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098762
Interaction Network
Interactions Browser links
Dcr-1 network
loqs network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0187593-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dicer-1

bait
loqs 

R3D1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

loqs coimmunoprecipitates with Dcr-1 but not Dcr-2.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0187593-10.EMSA.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dicer-1

bait

His tag

loqs 

r3d1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified proteins were incubated with [32]P labeled let-7 pre-miRNA and protein:RNA complex formation was monitored by gel shift.

Dcr-1 and loqs form a higher molecular weight RNA:protein complex with let-7 than just Dcr-1 alone. loqs alone does not bind let-7 pre-miRNA in this assay.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected insect cell system.

FBrf0187593-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

R3D1

bait

Dicer-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Dcr-1 coimmunoprecipitates with loqs (r3d1) but not r2d2.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0187593-6.CH.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dicer-1

neutral component

Dicer-1

neutral component

Dicer-1

neutral component
loqs 

R3D1

neutral component
loqs 
loqs 

R3D1

neutral component
loqs 

R3D1

neutral component
loqs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; proteins produced from endogenous genes.

Extract was fractionated by sequential ammonium sulfate precipitation and phenyl-sepharose, Mono S, Mono Q and hydroxyapatite chromatography.

Dcr-1 and loqs (L isoform) cofractionate together and with an miRNA-generating activity through several chromatographic steps.

FBrf0188210-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

Myc tag

Dcr-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Dcr-1 coimmunoprecipitates with both short and long isoforms of tagged transfected loqs. By contrast, endogenous long loqs isoforms preferentially coimmunoprecipitate with Dcr-1.

Dcr-1, but not Dcr-2, coimmunoprecipitates with loqs.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0188210-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

Dcr-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0188210-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dcr-1

bait
loqs 

Loqs

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

FBrf0188210-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dcr-1

bait
loqs 

Loqs

prey
loqs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0188216-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dcr-1

bait

FLAG tag

loqs 

Loqs

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0188216-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

Myc tag

Dcr-1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0188216-3.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

GST tag

Dcr-1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0200773-1.CS.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dcr-1

bait

His tag

loqs 

Loqs

prey

His tag

loqs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified proteins were mixed and subjected to sedimentation analysis.

When mixed with loqs, Dcr-1 migrates at a position consistent with the formation of a Dcr-1-loqs heterodimer.

Interaction in vitro; bait produced as a recombinant fusion protein in baculorvirus-infected Sf21 cell system; prey produced as a recombinant fusion protein in baculorvirus-infected Sf21 cell system.

FBrf0200773-4.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dcr-1

bait

FLAG tag

loqs 

Loqs

prey

His tag

loqs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Dcr-1 was cotransfected with the PA loqs isoform, the PB loqs isoform, or both simultaneously.

Dcr-1 can interact with either the PA or PB isoforms of loqs, but preferentially binds the PB isoform in competition experiments.

Source was cell extract of baculovirus-infected Sf21 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0200773-5.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Dcr-1

bait
loqs 

Loqs

prey
loqs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0200773-6.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

FLAG tag

loqs 

Dcr-1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
helicase domain
sufficient binding region
aa 1-690
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf21 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0200773-7.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

GFP tag

loqs 
loqs 
loqs 

Dcr-1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
helicase domain
sufficient binding region
aa 1-690
loqs 
dsRBD3 plus
sufficient binding region
aa 384-465
loqs 
PB specific linker
necessary binding region
aa 338-384

increase affinity of the interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Residues 338-384 of the loqs PB isoform (which are not present in the PA isoform) increase the affinity for Dcr-1.

FBrf0205160-3.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

Dcr-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0208906-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

TAP tag

loqs 
loqs 

Dcr-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Dcr-2 and Dcr-1 preferentially interact with different isoforms of loqs.

FBrf0209063-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

Myc tag

loqs 
loqs 

Dcr-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Dcr-2 and Dcr-1 preferentially interact with different loqs isoforms.

FBrf0210024-1.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

Dicer1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0210024-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

Dcr1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0210024-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

Myc tag

loqs 
loqs 

Dcr1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0215456-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

LOQS

bait

DCR-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0231028-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

GST tag

loqs 
loqs 
loqs 
loqs 
loqs 

dmDcr1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
loqs 
L2 loop plus dsRBD3 domain
sufficient binding region
aa 368-463

coordinates relative to loqs-PB

loqs 
L2 loop deletion
mutation decreasing interaction
aa 368-392

coordinates relative to loqs-PB

loqs 
L2 loop-residue 387
mutation decreasing interaction
aa 387

L387A, coordinates relative to loqs-PB

loqs 
dsRBD3 domain-residue 419
mutation decreasing interaction
aa 419

F419A, coordinates relative to loqs-PB

loqs 
dsRBD3 domain-residue 426
mutation disrupting interaction
aa 426

L426R, coordinates relative to loqs-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in Sf21 baculovirus-infected insect cell system.

The dsRBD3 domain of loqs is sufficient for only weak (~10%) binding of Dcr-1. The linker (L2 loop) of loqs is required in addition to the dsRBD3 domain for full binding to Dcr-1.

FBrf0231028-2.FCS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

GST tag

loqs 
loqs 

dmDcr1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
loqs 
L2 loop plus dsRBD3 domain
sufficient binding region
aa 368-463

coordinates relative to loqs-PB

loqs 
L2 loop deletion
mutation decreasing interaction
aa 368-392

coordinates relative to loqs-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in Sf21 baculovirus-infected insect cell system.

Deletion of the loqs linker (L2 loop) reduces binding to Dcr-1 ten-fold.

FBrf0231028-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dmDcr1

bait

Myc tag

loqs 

Loqs

prey

HA tag

loqs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
loqs 
dsRBD3 domain-residue 426
mutation decreasing interaction
aa 426

L426R, coordinates relative to loqs-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (10)