FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
side, beat-Ia
FlyBase ID
FBig0000098813
Interaction Network
Interactions Browser links
side network
beat-Ia network
Reported Interactions
FBrf0207941-1.BA.FM
Reference
Siebert et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
beat-Ia 

beat

neutral component

beat-myc

beat-Ia 

beat

neutral component

beat-myc

beat-Ia 

beat

neutral component

beat-myc

side 

side

neutral component
side 

side

neutral component
side 

side

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Siebert et al., 2009)

Cells in this assay were cotransfected with beat-Ia and side and formed large aggregates.

(Siebert et al., 2009)

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

(Siebert et al., 2009)
FBrf0207941-2.BA.FM
Reference
Siebert et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
beat-Ia 

beat

neutral component

beat-myc

beat-Ia 

beat

neutral component

beat-myc

beat-Ia 

beat

neutral component

beat-myc

side 

side

neutral component
side 

side

neutral component
side 

side

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Siebert et al., 2009)

Cells in this assay were transfected with either beat-Ia and side and the resulting cell populations were mixed to observe aggragation.

(Siebert et al., 2009)

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

(Siebert et al., 2009)
FBrf0207941-3.BA.FM
Reference
Siebert et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
beat-Ia 

beat

neutral component

beat-Cherry

beat-Ia 

beat

neutral component

beat-Cherry

beat-Ia 

beat

neutral component

beat-Cherry

side 

side

neutral component
side 

side

neutral component
side 

side

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Siebert et al., 2009)

Cells in this assay were transfected with either beat-Ia and side and the resulting cell populations were mixed to observe aggragation.

(Siebert et al., 2009)

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

(Siebert et al., 2009)
FBrf0207941-4.coIP.WB
Reference
Siebert et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
side 

Side

bait

GFP tag

beat-Ia 

Beat

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Siebert et al., 2009)
FBrf0207941-5.coIP.WB
Reference
Siebert et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
beat-Ia 

Beat

bait

GFP tag

side 

Side

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Siebert et al., 2009)
FBrf0222007-196.SPR
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
beat-Ia 

Beat-Ia

neutral component

His tag

beat-Ia 

Beat-Ia

neutral component

His tag

beat-Ia 
beat-Ia 

Beat-Ia

neutral component

His tag

beat-Ia 
side 

Side

neutral component

His tag

side 
side 

Side

neutral component

His tag

side 

Side

neutral component

His tag

side 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
beat-Ia 
extracellular domain
sufficient binding region
side 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced and purified from baculovirus-infected High Five cells.

(Ozkan et al., 2013)

Kd = 3.1E10-6 M

(Ozkan et al., 2013)
FBrf0222867-108.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
side 

side

bait

Fc tag

side 
beat-Ia 

beat-Ia

prey

alkaline phosphatase tag, COMP pentamerization motif

beat-Ia 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
side 
extracellular domain
sufficient binding region
beat-Ia 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
FBrf0222867-22.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
beat-Ia 

beat-Ia

bait

Fc tag

beat-Ia 
side 

side

prey

alkaline phosphatase tag, COMP pentamerization motif

side 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
beat-Ia 
extracellular domain
sufficient binding region
side 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
FBrf0255320-1.coIP.WB
Reference
Heymann et al., 2022
Description
physical association
Assay
Collection
Source/Stage
hypodermal muscle of A1-7 / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
beat-Ia 

beat-Ia

bait

HA tag

beat-Ia 
side 

side

prey

Cherry tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
beat-Ia 
immunoglobulin domain
necessary binding region
aa 140-427

coordinates relative to AA-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Heymann et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)