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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098814
Interaction Network
Interactions Browser links
Mer network
ex network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0125175-1.FW.TV
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

MER

bait

GST tag

Mer 
ex 

EX

prey

GST tag, biotin label

ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
C-terminal region
sufficient binding region
aa 522-635
ex 
CNTR domain
sufficient binding region
aa 1-399
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The ex CNTR domain binds to Mer but not Moe.

Bait protein was separated by SDS-PAGE and transferred to nitrocellulose, then incubated with biotinylated prey protein.

FBrf0247729-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

V5 tag

Mer 

Mer

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)