FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rho1, spir
FlyBase ID
FBig0000098846
Interaction Network
Interactions Browser links
Rho1 network
spir network
Reported Interactions
FBrf0191267-2.PD.A
Reference
Rosales-Nieves et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho

bait

GST tag

Rho1 
Rho1 
spir 

Spir

prey

[35]S label

spir 
spir 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rho1 
region
mutation increasing interaction
aa 14

G14V, mimics GTP-bound state

Rho1 
region
mutation decreasing interaction
aa 19

T19N, mimics GDP-bound state

spir 
WH2 domains
sufficient binding region
aa 366-491

\'D3\', relative to spir-PD

spir 
Spire-box, FYVE, JNK-binding domains
sufficient binding region
aa 190-626

\'C3\', relative to spir-CD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Rosales-Nieves et al., 2006)
FBrf0208460-10.coIP.WB
Reference
Liu et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait
spir 

Spir

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Liu et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)