Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.
Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.
Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.
Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.
I83D coordinates relative to DIP-α-PA
Interaction in vitro; bait produced as a recombinant fusion protein in HEK293F cells; prey produced as a recombinant fusion protein in HEK293F cells.
Kd = 1.67uM
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculoviral Sf9 cell system.
Interaction in vitro; bait derived from conditioned medium of transfected S2 cell line; prey derived from conditioned medium of transfected S2 cell line.
L93G,Q142E,V144K, coordinates relative to dpr10-PA
Q138D, , coordinates relative to dpr10-PA
Interaction in vitro; bait purified from transfected HEK293F cells; prey purified from transfected HEK293F cells.
Kd = 1.5uM, average of 5 independent measurements
V144K, Q142E, G99D
Typo in Fig1A for the interaction Kd of wild-type DIP-α and dpr10 Q142M; the correct value is demonstrated in the graph, text and Fig A,B. DIP-α wild type and mutant interaction Kds with wild type dpr10 : wild type 1.36um, G74A 0.91um, Q81Q 31.8, K81Q and G74S 68.0um; dpr10 mutant interaction Kds with wild type DIP-α : Q142M 0.19um, Q138D 27.8um, V144K 11.3, V144K, Q142E and G99D 50um; DIP-α G74A interaction with dpr10 Q142M Kd: 0.104.
Interaction in vitro; bait and prey produced from transfected constructs in HEK293F cells.
