RISC activity was extracted from ribosomes, then fractionated by gel filtration and ion exchange chromatography.

AGO2, Fmr1 and vig cofractionate as part of the RISC complex.

Source was cell extract of S2 cell line; proteins produced from endogenous genes.

The interaction is not affected by RNaseA treatment.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

The interaction is not affected by RNaseA treatment.

RISC activity was extracted from ribosomes, then fractionated by gel filtration and Source Q chromatography.

AGO2, Tudor-SN, Fmr1 and vig cofractionate as part of the RISC complex.

Source was cell extract of S2 cell line; proteins produced from endogenous genes.