Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

brm, mor, dalao, Hsc70-4, Bap60, Bap55, Snr1 and Bap47 (actin) were identified as components of the Brahma complex.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before pull down of His tagged brm.