The GTP-bound form of Rab30 binds CG3493, CG10703, cbs, CG3532, GM130 and BicD, but not Gmap.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

The GTP-bound form of Rab30 binds CG3493, CG7324, BicD, p115, CG10703, ik2 and Rep.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

BicD specifically binds the GTP-bound state of Rab30 (but not the GDP-locked state).

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract (endogenous gene).

Specificity S-score=12.78.

Constitutively active (GTP-locked) form of Rab was used for pull down. Parallel pull downs performed for several Rabs proteins in this study. Yield was measured using spectral counts. A specificity score (S score) was calculated, where greater weight was given to interactions seen with fewer baits.