FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
bora, aurA
FlyBase ID
FBig0000099074
Interaction Network
Interactions Browser links
bora network
aurA network
Reported Interactions
FBrf0192833-1.coIP.WB
Reference
Hutterer et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
bora 

Bora

bait

GFP tag

aurA 

AurA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hutterer et al., 2006)
FBrf0192833-2.PD.A
Reference
Hutterer et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bora 

Bora

bait

GST tag

bora 
bora 
aurA 

AurA

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bora 
N-terminal region
sufficient binding region
aa 1-209

\'Δ5\'

bora 
N-terminal region
necessary binding region
aa 1-67

\'Δ1\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Hutterer et al., 2006)
FBrf0192833-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aurA 

AurA

enzyme

His tag

aurA 

AurA

enzyme

His tag

bora 

Bora

enzyme target

MBP tag, [32]P label

bora 

Bora

enzyme target

MBP tag, [32]P label

bora 
bora 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)