FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Spc25, Nnf1b
FlyBase ID
FBig0000099096
Interaction Network
Interactions Browser links
Spc25 network
Nnf1b network
Reported Interactions
FBrf0200315-7.coIP.MS
Reference
Schittenhelm et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 10embryonic stage 12embryonic stage 11
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spc25 

Spc25

bait

RFP tag

Nnf1b 

Nnf1b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Schittenhelm et al., 2007)
FBrf0200394-37.coIP.MS
Reference
Przewloka et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Spc25 

dmSpc25R

bait

Protein A tag

Nnf1b 

dmNnf1R-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Przewloka et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)