FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Uba2, Sumo
FlyBase ID
FBig0000099241
Interaction Network
Interactions Browser links
Uba2 network
Sumo network
Reported Interactions
FBrf0132366-6.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Uba2 

DmUba2

enzyme

His tag

Uba2 

DmUba2

enzyme

His tag

Sumo 

DmSUMO-1

enzyme target

GST tag, [32]P label

Sumo 

DmSUMO-1

enzyme target

GST tag, [32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0144953-24.PD.WB
Reference
Shih et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

DmSmt3

bait

His tag

Sumo 
Uba2 

DmUba2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sumo 
C-terminally cleaved region
sufficient binding region
aa 1-88

coordinates relative to smt3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Shih et al., 2002)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)