Interaction in vitro; enzyme produced as a recombinant fusion protein in baculovirus-infected Sf9 cell system.
polo kinase is autophosphorylated.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.
Intramolecular interaction. The C-terminal polo-box domain of polo interacts with the N-terminal kinase domain.
Source was live HEK293T cells; fluorescence donor produced from tagged transfected construct; fluorescence acceptor produced from tagged transfected construct.
