FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ras85D, cno
FlyBase ID
FBig0000099351
Interaction Network
Interactions Browser links
Ras85D network
cno network
Reported Interactions
FBrf0202988-4.Y2H
Reference
Boettner and Van Aelst, 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ras85D 

Ras1

bait

fused to LexA DNA-binding domain

Ras85D 
cno 

Cno

prey

fused to GAL4 activation domain

cno 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ras85D 
N-terminal domain
mutation disrupting interaction

dominant-negative N17 form of Ras85D

cno 
N-terminal domain
sufficient binding region
aa 1-396

coordinates relative to cno-PE

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Boettner and Van Aelst, 2007)

Two-hybrid system: yeast LexA-BD/GAl4-AD

(Boettner and Van Aelst, 2007)
FBrf0223134-5.Y2H
Reference
Carmena et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ras85D 

RAS

bait

fused to GAL4 DNA-binding domain

cno 

CNO

prey

fused to GAL4 activation domain

cno 
cno 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cno 
Ras-associating, KIN, MYO and PDZ domains
sufficient binding region
aa 1-935

full binding

cno 
Ras-associating domains
sufficient binding region
aa 1-355

moderate binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Carmena et al., 2006)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Carmena et al., 2006)

The activated form of Ras85D (Ras[V12]) was used in this assay.

(Carmena et al., 2006)
FBrf0223134-8.coIP.A
Reference
Carmena et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cno 

Cno

bait

Myc tag, [35]S label

Ras85D 

Ras1[V12]

prey

HA tag, [35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Ras85D and dsh bind competitively to cno. Though cno binds more strongly to Ras85D, increasing amounts of dsh out-compete this interaction.

(Carmena et al., 2006)

Source was embryos of wild-type fly line; bait produced by in vitro translation; prey produced and labeled by in vitro translation.

(Carmena et al., 2006)

The activated form of Ras85D (Ras[V12]) was used in this assay.

(Carmena et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)