FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdc42, gek
FlyBase ID
FBig0000099396
Interaction Network
Interactions Browser links
Cdc42 network
gek network
Reported Interactions
FBrf0099374-1.Y2H
Reference
Luo et al., 1997
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 15late extended germ band stageembryonic stage 12embryonic stage 11embryonic stage 8embryonic stage 9early extended germ band stageembryonic stage 13embryonic stage 14embryonic stage 10extended germ band stageembryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Dcdc42

bait

fused to LexA DNA-binding domain

Cdc42 
Cdc42 
Cdc42 
gek 

Gek

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cdc42 
region
mutation increasing interaction
aa 12

G12V, disrupts GTPase activity to mimic activated form

Cdc42 
region
mutation disrupting interaction
aa 17

T17N, dominant negative form

Cdc42 
effector domain
mutation disrupting interaction
aa 35

T35A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Luo et al., 1997)

Lipid anchoring motif of Cdc42 was removed to permit nuclear targeting of LexA fusion protein.

(Luo et al., 1997)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Luo et al., 1997)

Two independent Cdc42-interacting clones were isolated representing gek cDNAs.

(Luo et al., 1997)

gek binds the activated form of Cdc42.

(Luo et al., 1997)
FBrf0099374-2.PD.A
Reference
Luo et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Dcdc42

bait

GST tag

Cdc42 
Cdc42 
gek 

Gek

prey

[35]S label

gek 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cdc42 
region
mutation increasing interaction
aa 12

G12V, disrupts GTPase activity to mimic activated form

Cdc42 
effector domain
mutation disrupting interaction
aa 35

T35A

gek 
CRIB motif
mutation disrupting interaction
ISAP

\'Δ\'ISP

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Luo et al., 1997)

gek binds the activated form of Cdc42, but not to another GTPase Rac1.

(Luo et al., 1997)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)