FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
aurA, cnn
FlyBase ID
FBig0000099466
Interaction Network
Interactions Browser links
aurA network
cnn network
Reported Interactions
FBrf0162192-1.Y2H
Reference
Terada et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aurA 

Aurora-A

bait

fused to GAL4 DNA-binding domain

cnn 

CNN

prey

fused to GAL4 activation domain

cnn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
C-terminal region
sufficient binding region
aa 665-1148
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Terada et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Terada et al., 2003)

Two aur-interacting clones were isolated represeting cnn cDNAs.

(Terada et al., 2003)
FBrf0162192-2.coIP.WB
Reference
Terada et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
cnn 

CNN

bait

HA tag

aurA 

AurA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

cnn binds to aur (Aurora-A) but not ial (Aurora-B).

(Terada et al., 2003)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Terada et al., 2003)
FBrf0162192-3.PD.A
Reference
Terada et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aurA 

AurA

bait

His tag

cnn 

CNN

prey
cnn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
C-terminal region
sufficient binding region
aa 665-1148
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Terada et al., 2003)
FBrf0233282-69.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aurA 

Aurora A

bait

fused to GAL4 DNA-binding domain

cnn 

Cnn

prey

fused to GAL4 activation domain

cnn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
N-terminal region
sufficient binding region
aa 1-365

coordinates relative to cnn-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0233282-70.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

Cnn

bait

fused to GAL4 DNA-binding domain

cnn 
cnn 
aurA 

Aurora A

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
N-terminal region
sufficient binding region
aa 1-365

coordinates relative to cnn-PA

cnn 
C-terminal region
sufficient binding region
aa 698-1148

coordinates relative to cnn-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)