Rab11 was loaded with GTPγS (non-hydrolyzable) or GDP.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Rab11 was pre-loaded with GTPγS-Mg2+
Q70L, coordinates relative to Rab11-PA
S25N, coordinates relative to Rab11-PA
Rab11 locked in the GTP bound conformation was pre-loaded with GTPγS-Mg2+; GDP-restricted Rab11 was pre-loaded with GDP-Mg2+; Nucleotide free Rab11 was pre-loaded with EDTA which caused a disruption in the interaction.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Q70L, coordinates relative to Rab11-PA
N659A, coordinates relative to Sec15-PA
M663A, coordinates relative to Sec15-PA
F667A, coordinates relative to Sec15-PA
Q664A, coordinates relative to Sec15-PA
Q664E, coordinates relative to Sec15-PA
Rab11 locked in the GTP bound conformation was pre-loaded with GTPγS-Mg2+.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Rab11 locked in the GTP bound conformation was pre-loaded with GTPγ.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct.
