Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

It is suggested that lmgA and mr jointly create a binding site for vih since pairwise interaction of vih to lmgA or mr could not be detected.

Three-hybrid system: yeast LexA/GAL4-AD.

This assay specifically detects interactions involving all three components.