Ubiquitin chains further characterized using antibodies specific for K48 or K63 linkages.

Cleavage of grim by Ice and/or Nc at residue 132 removes the site of ubiquitination at residue 136 and increases the stability of the grim protein. Mutation of the cleavage site increases the amount of ubiquitinated grim observed.

grim ubiquitin chains are composed of K48-based linkages of four or fewer ubiquitin units.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Ubiquitin chains detected using antibodies specific for K48 or K63 linkages.

Cleavage of grim by Ice and/or Nc at residue 132 removes the site of ubiquitination at residue 136 and increases the stability of the grim protein. Mutation of the cleavage site increases the amount of ubiquitinated grim observed.

grim ubiquitin chains are composed of K48-based linkages.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.