FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
polo, gwl
FlyBase ID
FBig0000099518
Interaction Network
Interactions Browser links
polo network
gwl network
Reported Interactions
FBrf0222183-1.coIP.MS
Reference
Wang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gwl 

Gwl

bait

Protein A tag

polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Wang et al., 2013)
FBrf0222183-2.coIP.WB
Reference
Wang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gwl 

Gwl

bait

Protein A tag

polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Wang et al., 2013)
FBrf0222183-3.coIP.WB
Reference
Wang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gwl 

Gwl

bait

Protein A tag

polo 

Polo

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

(Wang et al., 2013)
FBrf0222183-4.coIP.WB
Reference
Wang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
polo 

Polo

bait

Protein A tag

gwl 

Gwl

prey

Myc tag

gwl 
gwl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gwl 
central region
sufficient binding region
aa 400-675

contains both nuclear localization signals

gwl 
N-terminal kinase domain
sufficient binding region
aa 1-245

weak binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2013)

polo does not require the phoshpo binding activity of its \'Polo-box domain\' to interact with gwl, suggesting that phosphorylation of gwl is not required for this interaction.

(Wang et al., 2013)
FBrf0222183-6.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
polo 

Polo

enzyme

His tag

polo 

Polo

enzyme

His tag

gwl 

Gwl

enzyme target

[32]P label, GST tag

gwl 

Gwl

enzyme target

[32]P label, GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)