The GTP-bound form of Arl1 binds CG3493, CG10703 and cbs.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

CG10703 binds the GTP-bound form of Arl1 or Rab30. Binding of CG01703 to Rab30 is stronger than binding to Arl1.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

From a panel of 27 small G proteins tested, CG10703 interacted with Arl1, Rab6, Rab19 and Rab30.

CG10703 binds to the activated (GTP-locked) forms of small G proteins (Q-to-L mutation).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

GCC88 interacts preferentially with a version of Arl1 that mimics the GTP-bound state, over one that mimics the GDP-bound state.

GCC88 interacts with Arl1, but not with Arf79F (Arf1) or Arf102F (Arf4) in this assay.

The N-terminal amphipathic helix of Arf protein was replaced with 10-His residues. His-tagged bait Arf protein was then used to coat the surface of liposomes containing low levels of a lipid with Ni-nitrilotriacetic acid (NTA) head group. Bait-coated liposomes are mixed with cell lysate, then liposomes are purified by centrifugation. This allows for the identification of protein interactions that occur preferentially or exclusively at the surface of a lipid bilayer.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

GCC88 interacts preferentially with a version of Arl1 that mimics the GTP-bound state, over one that mimics the GDP-bound state.

The N-terminal amphipathic helix of Arf protein was replaced with 10-His residues. His-tagged bait Arf protein was then used to coat the surface of liposomes containing low levels of a lipid with Ni-nitrilotriacetic acid (NTA) head group. Bait-coated liposomes are mixed with cell lysate, then liposomes are purified by centrifugation. This allows for the identification of protein interactions that occur preferentially or exclusively at the surface of a lipid bilayer.