FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hsc70-3, yki
FlyBase ID
FBig0000099755
Interaction Network
Interactions Browser links
Hsc70-3 network
yki network
Reported Interactions
FBrf0223213-11.coIP.WB
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Hsc70-3 

HSC70-3

bait

GFP tag

yki 

yki

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kwon et al., 2013)
FBrf0224345-194.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

Hsc70-3 

Hsc70-3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0254684-1.PLA
Reference
Yang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

neutral component

MYC tag

yki 

yki

neutral component

MYC tag

yki 

yki

neutral component

MYC tag

Hsc70-3 

Bip

neutral component

HA tag

Hsc70-3 

Bip

neutral component

HA tag

Hsc70-3 

Bip

neutral component

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic construct.

(Yang et al., 2022)
FBrf0254684-2.PLA
Reference
Yang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

neutral component

FLAG tag

yki 

yki

neutral component

FLAG tag

yki 

yki

neutral component

FLAG tag

Hsc70-3 

Bip

neutral component

HA tag

Hsc70-3 

Bip

neutral component

HA tag

Hsc70-3 

Bip

neutral component

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic construct.

(Yang et al., 2022)
FBrf0254684-3.coIP.WB
Reference
Yang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
eye / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

FLAG tag

Hsc70-3 

Bip

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult eyes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

(Yang et al., 2022)
FBrf0254684-4.coIP.WB
Reference
Yang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
eye / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hsc70-3 

Bip

bait

HA tag

yki 

yki

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Text states experiments in Fig3O are reciprocal; actual figure has a typo for the IP tag.

(Yang et al., 2022)

Source was adult eyes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

(Yang et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)