Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).
Two-hybrid system: yeast LexA-BD/B42-AD.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42-AD.
Source was adult ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
In the presence of RNase during immunoprecipitation this interaction was still observed, indicating that it is not mediated through RNA in the RNP complex; may be direct.
Disrupts interaction only if aa 334-416 is also deleted.
Disrupts interaction only if aa 1-146 is also deleted.
S7E requires the mutation T135E to decrease the interaction.
T135E requires the mutation S7E to decrease the interaction.
S4E requires the mutation S7E to decrease the interaction.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
aret S7E requires the mutation aret T135E to decrease the interaction while aret T135E also requires the mutation aret S7E to decrease the interaction. Additionally, aret S4E requires the mutation aret S7E to decrease the interaction. Together, aret S7E, T135E, and S4E greatly reduce the interaction.
cup was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
Source was live S2R+ cells; bait and prey produced from endogenous genes.
