FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nrg, Ank2
FlyBase ID
FBig0000100387
Interaction Network
Interactions Browser links
Nrg network
Ank2 network
Reported Interactions
FBrf0221368-1.coIP.WB
Reference
Enneking et al., 2013
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrg 

Nrg

bait
Nrg 
Ank2 

Ank2

prey
Ank2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Enneking et al., 2013)
FBrf0221368-2.coIP.WB
Reference
Enneking et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ank2 

Ank2-L

bait

EGFP tag

Nrg 

Nrg180

prey

HA tag

Nrg 
Nrg 
Nrg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nrg 
tyrosine phosphorylation site
mutation decreasing interaction
aa 1234aa 1234

Y1234F, 30% decrease

Y1234D, 70% decrease

Nrg 
tyrosine phosphorylation site
mutation disrupting interaction
aa 1234

Y1234A, 90% decrease

Nrg 
Ankyrin interacting motif
necessary binding region
FIGQY

ΔFIGQY

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Enneking et al., 2013)
FBrf0255277-3.coIP.WB
Reference
Chen et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ank2 

Ank2

bait

V5 tag

Ank2 
Nrg 

Nrg180

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ank2 
common exon fragment
sufficient binding region
aa 1-1126
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)