FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ksr, Raf
FlyBase ID
FBig0000100606
Interaction Network
Interactions Browser links
ksr network
Raf network
Reported Interactions
FBrf0224702-256.coIP.MS
Reference
Friedman et al., 2011
Description
physical association
Assay
Collection
RTK-Ras-ERK_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
ksr 

ksr

bait

TAP tag

Raf 

phl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Friedman et al., 2011)

Overall SAINT probability of interaction = 0.99.

(Friedman et al., 2011)
FBrf0260095-25.coIP.WB
Reference
Maisonneuve et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Raf 

Raf

bait

Pyo tag

ksr 

Ksr

prey

V5 tag

ksr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ksr 
C-lobe base domain residue 902
mutation decreasing interaction
aa 902

M902D; coordinates relative to ksr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Co-expression of Dsor1 or cnk-ave-Dsor1 enhances Raf-ksr interaction, which is abrogated by ksr M902D or cnk D195K mutation.

(Maisonneuve et al., 2024)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Maisonneuve et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)