FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pli, Myd88
FlyBase ID
FBig0000100749
Interaction Network
Interactions Browser links
Pli network
Myd88 network
Reported Interactions
FBrf0224392-3.coIP.WB
Reference
Ji et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Myd88 

MyD88

bait

Myc tag

Myd88 
Myd88 
Pli 

Pli

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myd88 
CTE domain
sufficient binding region
aa 385-537
Myd88 
CTE domain
necessary binding region
aa 385-537
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ji et al., 2014)
FBrf0224392-5.coIP.WB
Reference
Ji et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Myd88 

MyD88

bait

FLAG tag

Pli 

Pli

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction of Pli with Myd88 is not perturbed by knockdown of pll or tub.

(Ji et al., 2014)

Overexpression or knockdown of Pli increased or decreased K48-linked ubiquitination of Myd88, respectively.

(Ji et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ji et al., 2014)
FBrf0224392-7.coIP.WB
Reference
Ji et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Pli 

Pli

bait

FLAG tag

Myd88 

MyD88

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ji et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)