FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ci, Gug
FlyBase ID
FBig0000100752
Interaction Network
Interactions Browser links
ci network
Gug network
Reported Interactions
FBrf0223699-1.Y2H
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

fused to GAL4 DNA-binding domain

ci 
Gug 

Atro

prey

fused to GAL4 activation domain

Gug 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal region
sufficient binding region
aa 1-440
Gug 
C-terminal region
sufficient binding region
aa 1727-1986
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Zhang et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Zhang et al., 2013)
FBrf0223699-10.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Gug 

Atro

bait

Myc tag

ci 

Ci

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tagged Rpd3-T7, Gug-Myc and ci-FLAG were co-transfected and subjected to sequential FLAG then Myc immunoprecipitation to test for ternary complexes.

(Zhang et al., 2013)

Based on two-step coimmunoprecipitation, ci, Gug and Rpd3 exist in a larger complex togther.

(Zhang et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)
FBrf0223699-2.Y2H
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

fused to GAL4 DNA-binding domain

ci 
Gug 

Atro

prey

fused to GAL4 activation domain

Gug 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal region
sufficient binding region
aa 1-150aa 104-212aa 212-268
Gug 
C-terminal region
sufficient binding region
aa 1727-1986
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Zhang et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhang et al., 2013)

Gug interacts with several non-overlapping N-terminal fragments of ci.

(Zhang et al., 2013)
FBrf0223699-3.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Gug 

Atro

bait

FLAG tag

Gug 
ci 

Ci

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gug 
C-terminal region
sufficient binding region

\'AtroC\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)
FBrf0223699-4.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

FLAG tag

Gug 

Atro

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)
FBrf0223699-5.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

Myc tag

ci 
Gug 

Atro

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal region
sufficient binding region
aa 1-440

\'CiN\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)
FBrf0223699-6.PD.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

GST tag

ci 
Gug 

Atro

prey

Myc tag

Gug 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal region
sufficient binding region
aa 76-367
Gug 
C-terminal region
sufficient binding region

\'AtroC\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Zhang et al., 2013)
FBrf0223699-8.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

FLAG tag

Gug 

Atro

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tagged Rpd3-T7, Gug-Myc and ci-FLAG were co-transfected and subjected to sequential FLAG then Myc immunoprecipitation to test for ternary complexes.

(Zhang et al., 2013)

Based on two-step coimmunoprecipitation, ci, Gug and Rpd3 exist in a larger complex togther.

(Zhang et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)