FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ci, HDAC1
FlyBase ID
FBig0000100840
Interaction Network
Interactions Browser links
ci network
HDAC1 network
Reported Interactions
FBrf0223699-7.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

FLAG tag

HDAC1 

Rpd3

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tagged Rpd3-T7, Gug-Myc and ci-FLAG were co-transfected and subjected to sequential FLAG then Myc immunoprecipitation to test for ternary complexes.

(Zhang et al., 2013)

Based on two-step coimmunoprecipitation, ci, Gug and Rpd3 exist in a larger complex togther.

(Zhang et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)