FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000100893
Interaction Network
Interactions Browser links
Ced-12 network
msk network
Reported Interactions
FBrf0223327-1.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msk 

Dim7

bait

GST tag

Elmo

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
necessary binding region

contains PH domain, EAD domain and PxxP motif

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

FBrf0223327-2.PD.WB
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msk 

Dim7

bait

GST tag

Elmo

prey

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic larval extract.

Tagged Ced-12 was expressed in the larval musculature.

FBrf0223327-3.coIP.WB
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Elmo

bait

YFP tag

msk 

Dim7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Tagged Ced-12 was expressed in the larval musculature, and pulled down using anti-Ced-12 antibody.

FBrf0223327-4.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Elmo

bait
msk 

Dim7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0223327-5.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msk 

Dim7

bait

GST tag

Elmo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

FBrf0223327-6.coIP.WB
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Elmo

bait
msk 

Dim7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0223327-7.PLA.TV
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Elmo

neutral component

Elmo

neutral component

Elmo

neutral component
msk 

Dim7

neutral component
msk 

Dim7

neutral component
msk 

Dim7

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fixed embryos; proteins produced from endogenous genes.

Interaction in situ was determined using the \'Duolink\' proximity ligation assay, which uses oligonucleotide technology to detect the presence of two distinct antigens within 40 nm of each other.

FBrf0223327-8.PLA.TV
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Elmo

neutral component

Elmo

neutral component

Elmo

neutral component
msk 

Dim7

neutral component
msk 

Dim7

neutral component
msk 

Dim7

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fixed larvae; proteins produced from endogenous genes.

Interaction in situ was determined using the \'Duolink\' proximity ligation assay, which uses oligonucleotide technology to detect the presence of two distinct antigens within 40 nm of each other.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)