FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dachs, Zyx
FlyBase ID
FBig0000100914
Interaction Network
Interactions Browser links
dachs network
Zyx network
Reported Interactions
FBrf0213900-1.coIP.WB
Reference
Rauskolb et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Zyx 

Zyx

bait

FLAG tag

Zyx 
Zyx 
dachs 

d

prey

V5 tag

dachs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Zyx 
N-terminal region
sufficient binding region
aa 2-385

coordinates relative to Zyx-PB

Zyx 
LIM domains
sufficient binding region
aa 386-585

coordinates relative to Zyx-PB, binds more strongly than full-length or N-terminal region.

dachs 
motor domain
sufficient binding region
nt 86-3216
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rauskolb et al., 2011)

Zyx, d, and wts thought to form trimeric complex. Interactions captured pairwise.

(Rauskolb et al., 2011)
FBrf0213900-2.coIP.WB
Reference
Rauskolb et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
dachs 

d

bait

V5 tag

dachs 
Zyx 

Zyx

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dachs 
motor domain
sufficient binding region
nt 86-3216
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rauskolb et al., 2011)

Zyx, d, and wts thought to form trimeric complex. Interactions captured pairwise.

(Rauskolb et al., 2011)
FBrf0223842-2.coIP.WB
Reference
Cao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dachs 

Myo20

bait

FLAG tag

dachs 
dachs 
Zyx 

Zyx102

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dachs 
interaction domain
sufficient binding region
aa 1-1063

coordinates relative to d-PE isoform

dachs 
N-terminal region
necessary binding region
aa 1-126

coordinates relative to d-PE isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and purified as a recombinant fusion protein in baculovirus-infected Sf9 cell system; prey produced and purified as a recombinant fusion protein in baculovirus-infected Sf9 cell system.

(Cao et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)