Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

rdx interacts with cal1, but not with cid (CENP-A) in yeast two hybrid assays. This suggests that cal1 recruits cid to rdx complexes.

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

rdx binds cal1, and depletion of rdx leads to a proteasome-dependent decrease in the level of cal1 protein.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Neither of the MATH or BTB domains of rdx were sufficient for cal1 binding.

Two-hybrid system: yeast LexA-BD/GAL4-AD