FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Moe, conu
FlyBase ID
FBig0000101201
Interaction Network
Interactions Browser links
Moe network
conu network
Reported Interactions
FBrf0223340-1.Y2H
Reference
Neisch et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stageadult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Moe 

Moe

bait

fused to LexA DNA-binding domain

Moe 
conu 

Conu

prey

fused to GAL4 activation domain

conu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Moe 
N-terminal region
sufficient binding region
aa 1-544

\'Δ\'ACT, lacks F-actin binding domain

conu 
Moe interaction domain
sufficient binding region
aa 179-294aa 185-336
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from mixed embryo and adult head cDNA expression library).

(Neisch et al., 2013)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Neisch et al., 2013)

Five unique conu clones were recovered.

(Neisch et al., 2013)
FBrf0223340-2.coIP.WB
Reference
Neisch et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Moe 

Moe

bait

FLAG tag

Moe 
Moe 
conu 

Conu

prey

HA tag

conu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Moe 
FERM domain
sufficient binding region
aa 1-311
Moe 
FERM domain
necessary binding region
aa 1-298
conu 
Moe interaction domain
sufficient binding region
aa 185-294
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Neisch et al., 2013)

conu interacts with full length Moe, and more strongly with a form of Moe lacking the F-actin binding domain (\'ΔACT\').

(Neisch et al., 2013)
FBrf0223340-3.coIP.WB
Reference
Neisch et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Moe 

Moe

bait

FLAG tag

Moe 
conu 

Conu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Moe 
N-terminal region
sufficient binding region
aa 1-544

\'Δ\'ACT, lacks F-actin binding domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Neisch et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)