FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hmr, Lhr
FlyBase ID
FBig0000101203
Interaction Network
Interactions Browser links
Hmr network
Lhr network
Reported Interactions
FBrf0223436-1.coIP.WB
Reference
Thomae et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Lhr 

LHR

bait
Hmr 

HMR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Thomae et al., 2013)
FBrf0223436-11.coIP.MS
Reference
Thomae et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait

FLAG tag, HA tag

Lhr 

LHR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Thomae et al., 2013)
FBrf0223436-12.coIP.MS
Reference
Thomae et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Lhr 

LHR

bait

Myc tag

Hmr 

HMR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Thomae et al., 2013)
FBrf0223436-3.coIP.WB
Reference
Thomae et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait
Lhr 

LHR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Thomae et al., 2013)
FBrf0224481-1.coIP.WB
Reference
Satyaki et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lhr 

Lhr

bait

HA tag

Hmr 

Hmr

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Satyaki et al., 2014)
FBrf0224481-2.coIP.WB
Reference
Satyaki et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hmr 

Hmr

bait

FLAG tag

Lhr 

Lhr

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Satyaki et al., 2014)
FBrf0224481-5.Y2H
Reference
Satyaki et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lhr 

Lhr

bait

fused to DNA-binding domain

Hmr 

Hmr

prey

fused to activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Satyaki et al., 2014)

Two-hybrid system: yeast

(Satyaki et al., 2014)
FBrf0224481-6.Y2H
Reference
Satyaki et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hmr 

Hmr

bait

fused to activation domain

Lhr 

Lhr

prey

fused to DNA-binding domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Satyaki et al., 2014)

Two-hybrid system: yeast

(Satyaki et al., 2014)
FBrf0250942-1.coIP.MS
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait
Lhr 

LHR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of an S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lukacs et al., 2021)
FBrf0250942-13.coIP.MS
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait

Flag tag

Hmr 
Lhr 

LHR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hmr 
C-terminal domain residues 1242-1413
mutation disrupting interaction
aa 1242-1413

deletion; coordinates relative to Hmr-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Over-expression of Hmr and Myc-tagged Lhr did not affect interaction.

(Lukacs et al., 2021)

Source was nuclear extracts of an S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lukacs et al., 2021)
FBrf0250942-43.coIP.WB
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait

HA tag

Hmr 
Lhr 

LHR

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hmr 
C-terminal domain residues 1242-1413
necessary binding region
aa 1242-1413

deletion; coordinates relative to Hmr-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of an SF21 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lukacs et al., 2021)
FBrf0250942-44.coIP.WB
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait

Flag tag

Hmr 
Lhr 

LHR

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hmr 
C-terminal domain residues 1242-1413
necessary binding region
aa 1242-1413

deletion; coordinates relative to Hmr-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of an S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lukacs et al., 2021)
FBrf0250942-46.coIP.WB
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hmr 

HMR

bait

Flag tag

Hmr 
Lhr 

LHR

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hmr 
C-terminal domain residues 1242-1413
necessary binding region
aa 1242-1413

deletion; coordinates relative to Hmr-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of an S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lukacs et al., 2021)
FBrf0250942-7.coIP.MS
Reference
Lukacs et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Lhr 

LHR

bait
Hmr 

HMR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of an S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lukacs et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)