Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Co-transfection of Hrs with smo leads to increased levels of ubiquitinated smo.

Treatment of cells with hh-containing medium diminishes the Hrs-smo interaction and reduces the level of smo ubiquitination.

The interaction of Hrs with smo is not affected by overexpression/knockdown of Ubpy (a smo deubiquitinase), or by mutation of smo ubiquitination target sites.

The interaction of Hrs with smo is blocked by a phosphatase inhibitor, okadaic acid, and phosphomimetic smo mutations.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Treatment of cells with hh-containing medium diminishes the Hrs-smo interaction and reduces the level of smo ubiquitination.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

PKA/CK1 phosphorylation of smo and Hrs-smo binding are mutually antagonistic, in vitro.

Co-expression of Uch-L5 decreased the smo-Hrs interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.