FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Pak, Cdc42
FlyBase ID
FBig0000101281
Interaction Network
Interactions Browser links
Pak network
Cdc42 network
Reported Interactions
FBrf0087303-2.PD.A
Reference
Harden et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pak 

DPAK

bait

GST tag

Pak 
Cdc42 

Dcdc42

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pak 
p21 binding domain
sufficient binding region
aa 1-196
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Harden et al., 1996)

Pak binds to Rac1 and Cdc42, but not to Rho, Ras or Rab proteins tested.

(Harden et al., 1996)
FBrf0194289-3.PD.WB
Reference
Nahm et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pak 

Pak

bait

GST tag

Pak 
Cdc42 

Cdc42

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pak 
p21 binding domain
sufficient binding region
aa 77-151
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Nahm et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)