Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult head cDNA expression library).

22 loco clones recovered in the Gαo screen.

Gαo was matrix-immobilized on CNBr-activated sepharose, pre-loaded with GDP or GTPγS, then incubated with loco.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

loco binds to Gαo regardless of its nucleotide state.