FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO1, pasi2
FlyBase ID
FBig0000101698
Interaction Network
Interactions Browser links
AGO1 network
pasi2 network
Reported Interactions
FBrf0215185-17.coIP.PCR
Reference
Hong et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

HA tag

pasi2 

CG8121

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hong et al., 2009)
FBrf0220359-9.coIP.PCR
Reference
Pinder and Smibert, 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 4embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 3embryonic cycle 4embryonic cycle 9embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

AGO1 
pasi2 

CG8121

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
MID domain
mutation disrupting interaction
aa 657-694

K657A, K694A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Pinder and Smibert, 2013)

Binding of AGO1 to CG8121 mRNA is mediated by miRNA complementarity, and disrupted by mutations in AGO1 (K657A, K694A) that disrupt miRNA binding.

(Pinder and Smibert, 2013)

Embryo extracts were crosslinked, immunoprecipitated and the level of specific coimmunoprecipitated mRNAs was quantified by RT-qPCR.

(Pinder and Smibert, 2013)

Positive control.

(Pinder and Smibert, 2013)
External Crossreferences and Linkouts ( 0 )
References (2)